Consistent with our in vivo results, we found mt fission in pressurized RGC somas in vitro (Supplementary Figures S4a Quantitative analysis showed that the number of mitochondria was significantly increased to 1.5 in pressurized RGCs (n compared with non pressurized control (0.86 n (P Supplementary Figure 4b). However, there was no difference in mt volume density in cultured RGCs (Supplementary Figure S4b). The 3D tomographic reconstructions showed detailed mt membrane structure including the packing Real NFL Jerseys Wholesale China Cheap Online
arrangement, shape and density of the cristae (Supplementary Figure S4c). Most of the cristae in control cells have both tubular and lamellar compartments. However, some cristae are completely lamellar or completely tubular. In the example shown, both lamellar and tubular cristae extended transversely. In contrast to the control mitochondrion (Supplementary Figure S4c and Supplementary Movie S5), a few lamellar cristae are also arranged longitudinally in a pressurized mitochondria (Supplementary Figure S4c and Supplementary Movie S6).
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